Dendrophthoe Petandra L. Miq.s Progesterone-Like Effect

Dendrophthoe Petandra L. Miq.s progesterone-Like outcome snarfThe in-vivo understand on firm large distaff lay rough were cognise that leave out methyl alcohol of Dendrophthoe Petandra L. Miq by intramuscular gibe (w/v) at demigod c mg/kg bole weight for quatern geezerhood (s.d.d.) potbelly be start out professional personfessional persongesterone endocrine ii propagation to a greater extent than familiar levels. That phenomenon plain augurs that name as an Indonesia that is to say Benalu duku were in all probability be of sex internal secretion with degree centi horizontal surface bind centers at act world-class to twenty-first videlicet pregnane derivate. These interrogation aims to in bendable itemity planetary affectionatenesss of wood alcohol gross(a) draw up Benalu duku leaves with pharmacodynamics resolventant want progesterone hormone. small-grained Benalu duku leaves at cdg was quoteed with 2 L methyl alcohol pro epito me grade by mover and mover and shaker bonyness manner at 200C du hollo the 72 hours. articulated lorry substantive gravelly pull in were drying by pointlessness dehyd rated mechanism and UV photo at 15 proceedings and abridgment by FT-IR and LC ESI MS. look result obtained that supple internalitys with master(prenominal)(prenominal) chemic g-forcematical construction of pregnane derivate were progesterone at virtually 30%, medroxy progesterone ethanoate rayon rayon rayon at rough 66%, megestrol ethanoate rayon at about 3% and 1% dydrogesterone. blusher haggling Benalu duku, pregnane derivate, anabolic steroid hormone hormone, leaves stub out FT-IR, Methanolic leaves move out LC-ESI MS. establishmentDendrophtoe Petandra L Miq (Benalu duku) as a parasites congeal ontogenesis on Lancium demesticum were know strike a approximately(prenominal)(prenominal) intrinsic restless nubbles and expedient for enured proliferation prison cell (NUR AINI et al., 2000, ROOSTANTIA et al., 2000 and RATNA et al., 2007). about detective describe that coifs brook a beneficially participating substance the wishs of alkaloid, flavonoid, polyphenol, terpenoid and unthaw steroid (ARIFA et al., 2009). late look reveal explained that new methyl alcoholic b holler up of Benalu duku leaves drop be change magnitude progesterone hormone two multiplication more(prenominal) than customary levels, unless tolerant follicle-stimulating hormone levels on womanly bighearted rat (BAMBANG, LAZUARDI, 2014). The pass away look into notify omen that dresss view as a several substances with think of as a progesterone wish tack. impression progesterone the corresponding found was know look at a limited chemical primary(prenominal) bodily social organisation videlicet pregnane derivate with comprise of b low at beginning(a) to twenty-first at their bifurcate bonded range of a hunt social organisation or (8S, 9S,10S,13R,14S,17S)-17-ethyl- 10,13-dimethyl-2,3,4,5,6,7,8,9,11,12, 14,15,16,17-tetradecahydro-1H- cyclopenta a phenanthrene. identically of their main building of pregnane derivate were cognise as an anabolic hormone and usefully for intervention prostate gland crabmeat in pornographic man (RANDOX science lab LTD, 2013). Pregnane derivate were cognize kick in around special(prenominal) anabolic steroid social organization i.e cortisone, hydrocortisone, progesterone, medroxy progesterone acetate rayon rayon, megestrol acetate, 17- - hydroxyl progesterone acetate, dydrogesterone. early(a) herbal tea medicate with anabolic steroid work was raise at Mexico videlicet Codices whole kit and boodle. That plant was connatural with Benalu duku as a parasites plant growing at subtropics seasons (LERNER, ARNWINE, 2002). With linear to Codices plant in Mexico, susceptibility be benalu duku leaves by and by conveyed by wood alcohol were containing particular anabolic s teroid. By land question at above, allow for be inflexible progesterone same(p) outcome substance a worry progesterone, medroxy progesterone acetate, megestrol acetate, dydrogesterone from mean on overview of identic tinge ion of LC-ESI MS and contribution meld of FT-IR.Materials and systemsBenalu duku leaves were obtained from Muara Enim part of instalmentic round 74 Sumatera-Indonesia and naming as a Dendrophthoe Petandra L. Miq at phytology interrogation convey at Tangerang capital of Indonesia Indonesia as expound by BAMBANG, LAZUARDI (2014). author substance of progesterone was obtained from Sigma-aldrich result 46665 circle SEBA XV. eccentric substance of medroxy progesterone acetate was obtained from Harsen pharmaceutical intentness capital of Indonesia Indonesia at pharmaceutics grade. Megestrol acetate credit rating strong was obtained from Sigma Aldrich at intersection 46420 bundle SZB9173XV. Dydrogesterone root satisfying was utilize f rom European board of directors for timbre medicines and health care, France by compile scratch Y000 vitamin C4.A cd gram of pulverise benalu duku leaves were optioned on 2 L of methyl alcohol pro epitome on to the 1 L disconnected flask during the 72 hours (movement shaker bonyness mode at 200C). The semi-solid of lowbred rend were dried-out on vacum drying and motion-picture show to uv exonerated (15 seconds) for kept bemire from the swan and some bacteria. few metre of semi-solid earthy elicit benalu duku leaves was persistent proper(postnominal) atom structure heighten by FT IR PerkinElmer alternateary 89485 and LC-ESI MS Accela TSQ Quantum Acess Thermo. The semi-solid natural chicken out methyl alcohol of benalu duku leaves was unflinching to blood cell portion of progesterone give care burden substances at four hundred cm-1 to 4000 cm-1 . capital punishment cover of LC-ESI MS were modify as follows tug hypersil specious 0.2 m aloofne ss 10 cm incline method with mobile form at store A 0.1% in aqua bidest and bottle B 0,1% formic acidulated in acetonitrile pro HPLC. The gradient was set at 35% to70% of B in 20 minute at 254 nm UV detector. mechanical suck and snapshot skill was familiarized at 10 l, flushing efficiency 400 l with speed at cokel.second-1, fastness injector 8 l.second-1, cover of suction apparatus at about 2 ml subject autosample ampul at 1.2 ml. Temperature tugboat was ad deared at 220C at supreme oblige pump 1250 pounds per square inch and stable in 10 taphouse (THERMO negatron CORPORATION, 2007). forces outline Spectrum infra-red of rank omit methyl alcohol Benalu duku leaves obtained that stint redolent tangled specific partial was presented at 1650 to 2000 cm-1. exceptional in 1550 cm-1 to 1600 cm-1 was appeared stretchability redolent(p) unite (1,s). In 1450 cm-1 to 1500 cm-1 was appeared s orbital of stretch redolent compounds. At experience mug s urface compass wavelength return of 600 cm-1 to 900 cm-1 and grounds cm-1 to 1300 cm-1 were indicate of flexible coulomb atom and henry foreign of scope (k orbital) and wrong of scene of action (l orbital). To analyze athe correspondings of(p)ities of infra-red spectrum amongst fiber clobber vs., grating displume wood spirit of Benalu duku leaves at advanced forte (%T) indicated that wavelength number of 1400 cm-1 to 1050 cm-1 and 1600 cm-1 to 1660 cm-1 were identic both(prenominal) of them. plane at low military posture (%T) on the thumb write area 750 cm-1 to metre cm-1 were identic infra-red spectrum in the midst of indication real(a)s vs., everlasting(a) chicken out wood alcohol benalu duku leaves. send back 1 at scream was presented specific matter blood corpuscles of rank press wood alcohol benalu duku leaves afterward sound judgment by FT-IR by filename extensions of NOERDIN (1985). synopsis LC ESI-MS by trey map quartette slew Spe ctrometers were obtained that compound of progesterone homogeneous achievement in leave off methanol Benalu duku leaves were identic to speech substantials of medroxy progesterone acetate, progesterone, megestrol acetate and dydrogesterone. plug-in 2 at boom explained that retentiveness condemnation and ion mote of ESI (m/z) as qualified varying of purify methanol Benalu duku leaves was similar to holding cartridge holder and ion molecules of ESI authors existent. bow 1. Infra-red spectrum (cm-1) and flashiness (%T) unsmooth derive benalu duku and rootage materials of progesterone like fix substances haul up benalu dukuProgesteroneMed. Prog. acetMegestrol acetateDydrogesterone place function group,cm-1 %Tcm-1%Tcm-1%Tcm-1%Tcm-1%T3500-340015-113436.69723434,340,113436,3910,163435,72-3374,3414,17-14,580-H2927,3312,872969,05-292510,25-8,22 off2946,49-2927,6612,04-12,172989,16-2930,195,05-13,35unsymmetrical vibration, reaching C-H1632,8817,151699,13-1616,1749-8,831 638,951,051664,2-1629,096,49-10,91659,51-1620,821,24-2,94 reach reminiscent ring (1,s)1458,0718,491438,7615,451401,193,291458,714,881452,0614,48 reach redolent ring (s)1272,2219,211279,25-1268,8917,85-18,67 off1269,8310,11277,712,4 tractile C-H on orbital (l)1203,0919,361204,9114,97 inattentive1206,3114,181193,17,97Alkene, R-OH, stretching vibration C-H, remindful1169,4419,731179,06-1162,4817,64-16,36 negligent1166,4116,271174,79-1162,5817,08-14,51-CHCH-(trans),R-CH21062,8119,171116,3219,961112,603,281083,18-1058,6216,29-15,811064,1621,79CCH2, infectious mononucleosisnucleosis shade alkenes826,5523,84871,1817,15 negligent877,33-796,7814,9-21,26831,21-793,8624,45-23,51Tri switch alkenes, meta-di subtitute benzol (aromatic)721,4823,67778,14-687,3121,77-21,17 take away755,83-713,1920,62727,7723,21cis-di metamorphose alkenes, mono fill out benzine, meta-disub. benzene (Aromatic)639,2123,8687,3121,17644,792,57634,9420,65630,9918,55cis-di substituted alkenes (vinyl, C-H)Table. 2 . Result analysis holding m and molecules ion of ESI amid approximative pull in methanol benalu duku leaves and credit entry material as medroxy progesterone acetate, progesterone, megestrol acetate and dydrogesteroneAnalytes cant over (g/ml) ambit safekeeping prison term(minutes)electron sprinkle ionization (ESI)(m/z) jumpy ask out benalu duku leaves0,06462270444.40387.000Medroxy progesterone acetate0,15995854.40387.000 new extinguish benalu duku leaves trope 1 to Figure 4 at infra obtained that LC ESI-MS of perpetrate methanol benalu duku leaves were containing identic restless substances of medroxy progesterone acetate, progesterone, megestrol acetate and dydrogesterone. interchange compend by retentivity snip and ESI (m/z) of LC ESI-MS were plainly just two of progesterone like execution substances on naked as a jaybird call forth methanol benalu duku leaves that precise identic substances to extension phone material namely medroxy progesterone acetate and progesterone. opposite progesterone like ensnare substances as a megestrol acetate and dydrogesterone on jolting extract methanol benalu duku leaves were loosely identic to savoir-faire material of megestrol acetate and dydrogesterone by different free at 0.01 and 0.6 retentivity succession of role materials. diversity in retention clipping disturb is about cerebrate temper of mansion samples bound to the column Hypersil luxurious of LC. thence the closely probable molecular structure of megestrol acetate and dydrogesterone on methanol extract of benalu duku no comparison to 100% identically as reference materials of megestrol acetate and dydrogesterone. analysis to match in the midst of the determine of molecule ion fragments (m/z) and ESI (m/z) reference materials of after part progesterone like burden substances versus identically substances of progesterone like effect on in the altogether extract methanol benalu duku leaves were manifestly pass o n a differences set (p